产品编号:I-404
产品描述:Anti-Mouse IL-15
反应种属:mouse
实验方法:WB, ELISA., ICC
标记:
规格:500ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Interleukin-15 (IL-15)
Antigen Affinity Purified
(IL-T, MGC9721)
Polyclonal Antibody
Prod. No.: I-404
Host: Goat
Pkg. Size: 1mg, 100ug
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Mouse Interleukin-15 (IL-15) recognizes Mouse IL-15. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 15 (IL-15) is a novel cytokine that uses beta- and gamma-chain of IL-2R for signal transduction and is structurally similarity to IL-2. IL-15 is secreted by activated monocytes/macrophages following infection by virus.1 This cytokine induces cell proliferation of natural killer cells. This cytokine and interleukin 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. IL-15 can recruit and activate T lymphocytes in the synovial membrane, thereby contributing to Rheumatoid arthritis pathogenesis.2
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse IL-15 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (Frozen), N, WB
References
1. Yoshikai Y.et al. (1996) J. Immunol. 156: 663
2. Liew FY. et al. (1996) Nat. Med. 4: 643
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
