产品编号:I-259
产品描述:Anti-Mouse IL-7-Biotin
反应种属:mouse
实验方法:WB, ELISA.
标记:
规格:50ug
供应商:leinco
价格(RMB):5985.00
订购:
说明书:
Goat Anti-Mouse Interleukin-7 (IL-7)
Biotin
(Lymphopoietin 1, LP-1, pre-B Cell Factor)
Polyclonal Antibody
Prod. No.: I-259
Host: Goat
Pkg. Size: 1mg, 50ug
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Interleukin-7 (IL-7) recognizes Mouse IL-7. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 7 (IL-7) is a glycoprotein cytokine initially referred to as pre-B cell growth factor because of its growth-promoting activity for cells early in the B lymphocyte lineage. Subsequent to IL-7 gene cloning and expression, IL-7 was also found to influence the activity of cells in the T lineage.1 IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells. It also stimulates proliferation of all cells in the lymphoid lineage (B and T lymphocytes and NK cells). IL-7 is not only a potent grown stimulus for pre-B cells, but also plays a role in T cell activation.2 IL-7 has significant clinical and biomedical potential, such as cancer therapy and HIV infections.3
Known Species Cross Reactivity: Ms+
Immunogen
E. coli-Derived Recombinant Mouse Interleukin-7
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Mouse IL-7 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse IL-7 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Namen AE. et al. (1990) Int J Cell Cloning 1: 168
2. Reed SG. et al. (1989) J Exp Med. 169: 707
3. Prakash S. et al. (2008) Appl. of Biochem Biotechnol. 151: 93
Related Products
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TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human IL-7 (Leinco Prod. No.: I-189)
Anti-Mouse IL-7 (Antigen Aff. Pur.) (Leinco Prod. No.: I-166)
Anti-Human IL-7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-258)
Recombinant Rat IL-7 (Leinco Prod. No.: I-192)
Anti-Human IL-7 (Leinco Prod. No.: I-175)
Anti-Human IL-7 (Antigen Aff. Pur.) (Leinco Prod. No.: I-151)
Recombinant Mouse IL-7 (Leinco Prod. No.: I-209)
Human IL-7 ELISA Development Kit (Leinco Prod. No.: I-826)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
