产品编号:I-250
产品描述:Anti-Mouse IL-3-Biotin
反应种属:mouse
实验方法:WB, ELISA.
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Mouse Interleukin-3 (IL-3)
Biotin
(MCGF (Mast Cell Growth Factor), Multi-CSF, HCGF, P-Cell Stimulation Factor, MCGF, MGC79398, MGC79399, MULTI-CSF)
Polyclonal Antibody
Prod. No.: I-250
Host: Goat
Pkg. Size: 1mg, 50ug
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Interleukin-3 (IL-3) recognizes Mouse IL-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 3 (colony-stimulating factor, multiple), also known as IL3 is a member of a family of growth factors, that supports the proliferation and development of hematopoietic precursors.1 IL3 can improve the body's natural response to disease as part of the immune system. It is secreted by activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
Known Species Cross Reactivity: Ms+
Immunogen
E. coli-Derived Recombinant Mouse Interleukin-3
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Mouse IL-3 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse IL-3 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Laston D et al. (1998) Science 241: 1820
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human IL-3 Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-249)
Recombinant Rat IL-3 (Leinco Prod. No.: I-281)
Anti-Rat IL-3β (Antigen Aff. Pur.) (Leinco Prod. No.: I-343)
Anti-Mouse IL-3 (Antigen Aff. Pur.) (Leinco Prod. No.: I-163)
Anti-Human IL-3 (Antigen Aff. Pur.) (Leinco Prod. No.: I-147)
Human Interleukin-3 (IL-3) ELISA Development Kit (Leinco Prod. No.: I-285)
Recombinant Human IL-3 (Leinco Prod. No.: I-185)
Anti-Human IL-3 (Leinco Prod. No.: I-630)
Anti-Mouse IL-3 (Leinco Prod. No.: I-661)
Anti-Human IL-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-758)
Anti-Human IL-3 (Leinco Prod. No.: I-686)
Mouse IL-3 ELISA Development Kit (Leinco Prod. No.: I-286)
Recombinant Mouse IL-3 (Leinco Prod. No.: I-206)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
