产品编号:I-240
产品描述:Anti-Mouse IGF-I-Biotin
反应种属:mouse
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Mouse Insulin-like Growth Factor-I (IGF-I)
Biotin
(Somatamedin C, IGF-IA)
Polyclonal Antibody
Prod. No.: I-240
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Insulin-like Growth Factor-I (IGF-I) recognizes Mouse IGF-I. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse IGF-I (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Related Products
Anti-Human IGF-I-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-235)
PN:
Recombinant Human IGF-I (Leinco Prod. No.: I-180)
Anti-Human IGF-I (Antigen Aff. Pur.) (Leinco Prod. No.: I-142)
Recombinant Rat IGF-I (Leinco Prod. No.: I-524)
Anti-Human IGF-I (Leinco Prod. No.: I-649)
Anti-Mouse IGF-I (Leinco Prod. No.: I-709)
Recombinant Human IGF-I R (Leinco Prod. No.: I-841)
Recombinant Mouse IGF-I (Leinco Prod. No.: I-239)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
