产品编号:R1137
产品描述:Anti-Human sRANK-Biotin
反应种属:human
实验方法:FC, WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5985.00
订购:
说明书:
Goat Anti-Human Soluble Receptor Activator of NFkB (sRANK)
Biotin
(TNFRSF11A, CD265, ODFR, OFE, RANK, TRANCER)
Polyclonal Antibody
Prod. No.: R1137
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Soluble Receptor Activator of NFkB (sRANK) recognizes Human sRANK. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
RANK (Receptor Activator of Nuclear Factor κ B) is a member of the tumor necrosis factor receptor (TNFR) family, which is involved in the regulation of dendritic cell function.1 RANK is expressed on osteoclasts and their precursors, hematopoietic precursors, dendritic cells, and mammary epithelial precursors2 and is known as a regulator of development and activation of osteoclasts in bone remodeling.3 Activation of the RANK-RANKL signaling pathway is directly responsible for dramatic focal erosions that are observed in inflammatory arthritis and aseptic loosening of orthopaedic implants.4
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human sRANKR (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, FC, WB
References
1. Higashio K et al. (1998) Biochem Biophys Res Commun. 253: 395
2. Baron R et al. (2006) J clin Invest. 116: 1525
3. Murali R et al. (2004) J Biol Chem. 279: 8269
4. Looney RJ et al. (2004) J Musculoskelet Neuronal Interact 4: 276
Related Products
Anti-Human sRANK (Antigen Aff. Pur.) (Leinco Prod. No.: R1139)
Recombinant Human sRANK (Leinco Prod. No.: R142)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
