产品编号:P175

产品描述:Anti-Human PlGF-1-Biotin

反应种属:human

实验方法:WB

标记:

规格:50ug

供应商:leinco

价格(RMB):5700.00

订购:

说明书:

Goat Anti-Human Placenta Growth Factor-1 (PlGF-1)
Biotin
(PGF, PlGF)
Polyclonal Antibody
Prod. No.: P175
Host:         Goat
Pkg. Size: 1 mg, 50 µg
Storage:    -20°C to -70°C

Description
Specificity:
Goat Anti-Human Placenta Growth Factor-1 (PlGF-1) recognizes Human PlGF-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Placenta growth factor 1 (PlGF-1) a member of the vascular endothelial growth factor (VEGF) family, acts as a natural antagonist of VEGF.1 PlGF-1 is expressed in placental tissues, colon and mammary carcinomas. PlGF is active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. PlGF-1 may be a potential regulation target for the control of diabetic retinal and macular oedema.2

Immunogen
E. coli-Derived Recombinant Human PlGF
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Human PlGF-1 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human PlGF-1 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

References
1. Cao Y et al. (2002) Cancer Cell 1: 99
2. Behar-Cohen F et al. (2007) Diabetologia 50: 461

Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human PlGF-1 (Leinco Prod. No.: P167)
Anti-Human PlGF-1 (Antigen Aff. Pur.) (Leinco Prod. No.: R174)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.