产品编号:P135
产品描述:Anti-Human PF4-Biotin
反应种属:human
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Platelet Factor 4 (PF4)
Biotin
(Oncostatin A, Ironplact, CXCL4, MGC138298, SCYB4)
Polyclonal Antibody
Prod. No.: P135
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Platelet Factor 4 (PF4) recognizes Human PF4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. Due to these roles, it is predicted to play a role in wound repair and inflammation.1 PF4 is usually found in a complex with proteoglycan. PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with a splice variant of the chemokine receptor CXCR3, known as CXCR3B.2
Platelet factor-4 is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.3 PF4 is the antigen in heparin-induced thrombocytopenia, an idiosyncratic autoimmune reaction to the administration of the anticoagulant heparin.4
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human PF-4 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB,
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 100 ng/ml when used in conjunction with Anti-Human PF4 (Leinco Prod. No.: P247) as the capture antibody at 2 µg/ml and an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) for the detection of Human PF-4.
References
1. Poncz M et al. (1990) Blood 76: 336
2. Romagnani P et al. (2003) J Exp Med. 197: 1537
3 Eiseman et al. (1990)
4. Warkentin TE et al. (2007) N. Engl. J. Med. 356: 891
Related Products
Anti-Human PF4 (Leinco Prod. No.: P247)
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
Anti-Mouse PF4 (Leinco Prod. No.: C1444)
Anti-Mouse PF4 (Antigen Aff. Pur.) (Leinco Prod. No.: C1470)
Anti-Human PF4 (Antigen Aff. Pur.) (Leinco Prod. No.: C1479)
Anti-Mouse PF4-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1514)
Anti-Human PF4 (Antigen Aff. Pur.) (Leinco Prod. No.: P123)
Recombinant Human PF4 (Leinco Prod. No.: P127)
Recombinant Mouse PF4 (Leinco Prod. No.: P258)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
