产品编号:P173

产品描述:Anti-Human Persephin-Biotin

反应种属:human

实验方法:WB

标记:

规格:50ug

供应商:leinco

价格(RMB):5910.00

订购:

说明书:

Goat Anti-Human Persephin(PSP)Biotin(PSPN)
Polyclonal Antibody
Prod. No.: P173
Host:         Goat
Pkg. Size: 1 mg, 25 µg, 50 µg
Storage:    -20°C to -70°C

Description
Specificity:
Goat Anti-Human Persephin (PSP) recognizes Human PSP. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Persephin (PSP) is a secreted protein belonging to the glial cell line-derived neurotrophic factor (GDNF) family of the TGF-b superfamily. Just like factor GDNF and neurturin (NTN), PSP promotes the survival of ventral midbrain dopaminergic neurons and prevents their degeneration. Persephin also supports the survival of motor neurons.1 Persephin is expressed at very low levels in most tissues. It is synthesized throughout the nervous system and has both astroglial and neuronal origin.2

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human PSP (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB

References
1. Johnson EM et al. (1998) Neuron 20: 245
2. Krieglstein K. et al. (1998) J Neurosci Res. 53: 494

Related Products
Recombinant Human Persephin (Leinco Prod. No.: P136)
Anti-Human Persephin (Antigen Aff. Pur.) (Leinco Prod. No.: P172)
Recombinant Mouse Persephin (Leinco Prod. No.: P233)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.