产品编号:O105
产品描述:Anti-Human OPG-Biotin
反应种属:human
实验方法:WB, ELISA., ICC
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Osteoprotegerin (OPG)
Biotin
(TNFRSF11B, Osteoclast Inhibitory Factor (OCIF), MGC29565, TR1, OCIT)
Polyclonal Antibody
Prod. No.: O105
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Osteoprotegerin (OPG) recognizes Human OPG. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF) is a cytokine, which can inhibit osteoclast differentiation, activity, and survival.1 It is a member of the tumor necrosis factor (TNF) receptor superfamily.2 OPG may provide a pharmacological tool for osteoporotic and erosive bone disorders.3
Known Species Cross Reactivity: Hu+
Immunogen
Sf 21-Derived Recombinant Human Osteoprotegerin
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human OPG this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human OPG is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Kurihara H et al. (2002) Cytokine 18: 133
2. Schoppet M et al. (1980) Arteriosclerosis, Thrombosis, & Vascular Biol. 22: 549
3. Dunstan CR et al. (2001) J Bone Miner Res. 16: 348
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human Osteoprotegerin (Antigen Aff. Pur.) (Leinco Prod. No.: O104)
Recombinant Mouse Osteoprotegerin (Leinco Prod. No.: O122)
Anti-Human Osteoprotegerin (Leinco Prod. No.: O129)
Anti-Mouse Osteoprotegerin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: O132)
Anti-Mouse Osteoprotegerin (Leinco Prod. No.: O127)
Anti-Mouse Osteoprotegerin (Antigen Aff. Pur.) (Leinco Prod. No.: O125)
Recombinant Human Osteoprotegerin (Leinco Prod. No.: O102)
Recombinant Human Osteoprotegerin (Leinco Prod. No.: O137)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
