产品编号:N129
产品描述:Anti-Human NT-4-Biotin
反应种属:human
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Neurotrophin-4 (NT-4)
Biotin
(Neurotrophin 5, NT5, NTF5, Neutrophic Factor 5, Neutrophic Factor 4)
Polyclonal Antibody
Prod. No.: N129
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Neurotrophin-4 (NT-4) recognizes Human NT-4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Neurotrophin 4 (NT-4) is a novel neurotrophic factor1, the neurotrophins, that control survival and differentiation of vertebrate neurons. Several aspects distinguish NT-4 from other members of the neurotrophin family. It is the most divergent member and, in contrast to the other neurotrophins, its expression is ubiquitous and appears to be less influenced by environmental signals. NT-4 seems to have the unique requirement of binding to the low-affinity neurotrophin receptor for efficient signalling and retrograde transport in neurons.2 NT-4 controls survival and differentiation of mammalian neurons. It signals predominantly through the TrkB receptor tyrosine kinase. Both NT-4/5 and brain-derived neurotrophic factor may be physiological survival factors for facial motor neurons and may serve as therapeutic agents for motor neuron disease.3
Known Species Cross Reactivity: Hu+
Immunogen
Sf 21-Derived Recombinant Human NT-4
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Human NT-4 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human NT-4 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. H Persson et al. (1993) Development 117: 1345
2. Ibanez C. F. et al. (1996) Neurochemical Scie. 21: 787
3. Rosenthal A. et al. (1994) Proc Natl Acad Sci USA 91: 3304
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human NT-4 (Monoclonal Antibody) (Leinco Prod. No.: N115)
Anti-Human NT-4 (Leinco Prod. No.: N201)
Anti-Human NT-4 (Antigen Aff. Pur.) (Leinco Prod. No.: N114)
Recombinant Human NT-4 (Leinco Prod. No.: N118)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
