产品编号:N128

产品描述:Anti-Human NT-3-Biotin

反应种属:human

实验方法:WB, ELISA

标记:

规格:50ug

供应商:leinco

价格(RMB):5700.00

订购:

说明书:

Goat Anti-Human Neurotrophin-3 (NT-3)
Biotin
(Nerve Growth Factor-2 (NGF-2), HGN NTF3, HDNF, MGC129711)
Polyclonal Antibody
Prod. No.: N128
Host:         Goat
Pkg. Size: 1 mg, 50 µg
Storage:     -20°C to -70°C

Description
Specificity:
Goat Anti-Human Neurotrophin-3 (NT-3) recognizes Human NT-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Neurotrophin 3 (NT-3), also known as NTF3 is a basic protein found in neurons of the central nervous system. NT-3 selectively supports the survival of neuronal cell populations. It rapidly potentiate the spontaneous and impulse-evoked synaptic activity of developing neuromuscular synapses in culture and thus appears to be involved in the regulation of functions of developing synapses.1,2,3 NT-3 negatively modulates nerve growth factor (NGF) receptor expression and associated nociceptive phenotype in intact neurons, suggesting a beneficial role in treating aspects of neuropathic pain mediated by NGF. NT-3 is effective at suppressing thermal hyperalgesia associated with chronic constriction injury (CCI).4

Known Species Cross Reactivity: Hu+
Immunogen
Sf 21-Derived Recombinant Human NT-3
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Human NT-3 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human NT-3 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

References
1. Barde YA. et al. (1990) Progress in Growth Factor Res. 2: 237
2. Thoenen H. et al. (1991) Trends in Neurological Scienc. 14: 165
3. Vantini G. et al. (1992) Psychoneuroendocrinology 17: 401
4. Valerie M. K. Verge et al. (2005) J of Neuroscie. 25: 758

Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human NT-3 (Antigen Aff. Pur.) (Leinco Prod. No.: N113)
Anti-Human NT-3 (Leinco Prod. No.: N200)
Recombinant Human NT-3 (Leinco Prod. No.: N117)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.