产品编号:N148

产品描述:Anti-Human CLC-Biotin

反应种属:human

实验方法:WB

标记:

规格:50ug

供应商:leinco

价格(RMB):5700.00

订购:

说明书:

Goat Anti-Human Cardiotrophin-Like Cytokine (CLC)
Biotin
(NR6, B Cell Stimulating Factor-3 (BSF3), Novel Neurotrophin-1 (NNT1), CISS2, CLCF1)
Polyclonal Antibody
Prod. No.: N148
Host:         Goat
Pkg. Size: 1 mg, 50 µg
Storage:     -20°C to -70°C

Description
Specificity:
Goat Anti-Human Novel Neurotrophin-1 (NNT-1) recognizes Human NNT-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Cardiotrophin-like cytokine (CLC) also known as novel neurotrophin-1/B cell stimulating factor-3 (NNT-1/BSF-3) is a member of the interleukin (IL)-6 family of cytokines.1 CLC is expressed highly in spleen and peripheral leukocytes2 and is a target-derived factor required for the survival of specific pools of motoneurons.3 CLC shows neuroprotective effects on retinal ganglion cells (RGCs) in vivo and might be a treatment option for chronic neurodegenerative eye diseases such as glaucoma.4

Immunogen
E. coli-Derived Recombinant Human CLC
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB
Western Blotting: To detect Human NNT-1 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human NNT-1 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

References
1. Taga T et al. (2002) Cytokine 18: 1
2. Shi Y et al. (1999) Biochemical Biophysical Res Communications 262: 132
3. Oppenheim RW et al. (2003) J Neurosci. 23: 8854
4. Rong Wen et al. (2005) Graefe's Archive for Clinical and Experimental Ophthalmol. 243: 1036
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human CLC (Leinco Prod. No.: N138)
Anti-Human CLC (Antigen Aff. Pur.) (Leinco Prod. No.: N147)
Anti-Human CLC (Leinco Prod. No.: C1462)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.