产品编号:N144

产品描述:Anti-Human Nanog-Biotin

反应种属:human

实验方法:WB, ICC

标记:

规格:50ug

供应商:leinco

价格(RMB):6150.00

订购:

说明书:

Goat Anti-Human Nanog Biotin
Polyclonal Antibody
Prod. No.: N144
Host:          Goat
Pkg. Size: 1 mg, 50 µg
Storage:     -20°C to -70°C

Description
Specificity:
Goat Anti-Human Nanog recognizes Human Nanog. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Nanog, a homeobox transcription factor1, plays a key role in maintaining stem cell pluripotency by regulating the expression of genes critical to stem cell renewal and differentiation.2 In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm). Nanog may be useful in the immunohistochemical diagnosis of tumors. It is expressed in germ cells of the fetus and in some germ cell tumors of the gonads3 and central nervous system.4

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human Nanog (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB

References
1. Schöler HR.et al. (2003) Cell 113: 551
2. Pei D. et al. (2005) J Biol. Chem. 280: 1401
3. Rajpert-De Meyts E. et al. (2005) Histopathology 47: 48
4. Ligon KL. et al. (2006) Am J Surg Pathol. 30: 1613

Related Products
Anti-Human Nanog (Antigen Aff. Pur.) (Leinco Prod. No.: N143)
Recombinant Human Nanog (Leinco Prod. No.: N140)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.