产品编号:M129
产品描述:Anti-Human CCL22-Biotin
反应种属:human
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Chemokine (C-C Motif) Ligand 22 (CCL22)
Biotin
(MDC, A-152E5.1, ABCD-1, DC/B-CK, MGC34554, SCYA22, STCP-1)
Polyclonal Antibody
Prod. No.: M129
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Chemokine (C-C Motif) Ligand 22 (CCL22) recognizes Human CCL22. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-C motif) ligand 22, also known as CCL22 is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4.1 CCL22 selectively chemoattract Th2 cytokine-producing cells2,3 and acts as an important activator of eosinophils once thes cells have migrated into tissue.4
Immunogen
E. coli-Derived Recombinant Human MDC
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Human MDC this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human MDC is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Sozzani S. et al. (2001) Eur. J. Immunol. 31: 812
2. Elias CG. et al. (1998) J Immunol. 161: 5027
3. Gray PW. et al. (1997) J Exp Med. 185: 1604
4. Teixeira MM. et al. (2003) J Leuko Biol. 73: 356
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse CCL22-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M1112)
Anti-Mouse CCL22 (Antigen Aff. Pur.) (Leinco Prod. No.: M1111)
Recombinant Human CCL22 (Leinco Prod. No.: M171)
Anti-Human CCL22 (Leinco Prod. No.: M1092)
Anti-Human CCL22 (Antigen Aff. Pur.) (Leinco Prod. No.: M176)
Mouse CCL22 ELISA Development Kit (Leinco Prod. No.: M1113)
Recombinant Mouse CCL22 (Leinco Prod. No.: M202)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
