产品编号:M197
产品描述:Anti-Human MIP-3α-Biotin
反应种属:human
实验方法:WB, ELISA., ICC
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Macrophage Inflammatory Protein-3 Alpha (MIP-3α)
Biotin
(CKb4, Liver Activation Regulated Chemokine (LARC), MIP3A, SCYA20, ST38, Exodus-1)
Polyclonal Antibody
Prod. No.: M197
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Macrophage Inflammatory Protein-3 Alpha (MIP-3α) recognizes Human MIP-3α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Macrophage Inflammatory Protein-3 Alpha (MIP3A) also known as CCL20 or liver activation regulated chemokine (LARC) is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils.1 MIP3A is specifically expressed by the follicle-associated epithelia (FAE) covering intestinal Peyer's patches (PPs) and is the only known chemokine ligand for the chemokine receptor CCR6.2 MIP3-alpha is produced by intestinal epithelial cells (including cells associated with mucosal lymphoid tissues), by keratinocytes in inflamed skin, and by cells in liver and lung.3,4 It plays an important role in the inflammatory response of blood monocytes and tissue macrophages.
Known Species Cross Reactivity: Hu+
Immunogen
E. coli-Derived Recombinant Human CCL20
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human MIP-3α this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human MIP-3α is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Nomiyama H et al. (1997) J. Biol. Chem. 272: 5846
2. Kucharzik T. et al. (2005) J. Pathol. 166: 1647
3. Cook DN. et al. (2000) Immunity 12: 495
4. Farber JM. et al. (2002) J. Immunol. 168: 4871
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human MIP-3α (Antigen Aff. Pur.) (Leinco Prod. No.: M182)
Anti-Mouse MIP-3α (Antigen Aff. Pur.) (Leinco Prod. No.: M1297)
Recombinant Rat MIP-3α (Leinco Prod. No.: M1244)
Anti-Rat MIP-3α (Leinco Prod. No.: M1280)
Anti-Mouse MIP-3α (Leinco Prod. No.: M1285)
Anti-Rat MIP-3α (Antigen Aff. Pur.) (Leinco Prod. No.: C1468)
Anti-Rat MIP-3α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M1311)
Anti-Human MIP-3α-Biotin (Leinco Prod. No.: M1231)
Anti-Mouse MIP-3α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M1316)
Anti-Human MIP-3α (Leinco Prod. No.: M1093)
Recombinant Mouse MIP-3α (Leinco Prod. No.: M201)
Recombinant Human MIP-3α (Leinco Prod. No.: M167)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
