产品编号:I-246
产品描述:Anti-Human IL-1β-Biotin
反应种属:human
实验方法:
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Interleukin-1 Beta (IL-1β)
Biotin
(IL1B, IL-1, IL1-Beta, IL1F2)
Polyclonal Antibody
Prod. No.: I-246
Host: Goat
Pkg. Size: 1 mg, 50 µg
Description
Specificity:
Goat Anti-Human Interleukin-1 Beta (IL-1β) recognizes Human IL-1β. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 1 beta (IL1 beta) is a proinflammatory cytokine belonging to IL1 family that is synthesized as a precursor. The IL1 beta precursor form is not biologically active. It is expressed mainly by stimulated macrophages and monocytes.1,2 IL1 beta is known to be a mediator in inflammatory response and to be involved in a variety of cellular activities, proliferation, differentiation and apoptosis. It has been recently found that IL1 beta is a major cause of severe inflammation in the mouse model of Crohns disease which makes IL1 beta an important target for this disease.3
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human IL-1β (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, IHC (NBF/Par.), WB,
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human IL-1β this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-1β is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human IL-1β (Leinco Prod. No.: I-183).
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 ng/ml.
References
1. Dinarello CA et al. (1996) Blood 87: 2095
2. Yuan et al. (1993) Cell 75: 641
3. Shin Maeda et al. (2005) Science 4: 735
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human IL-1β (Leinco Prod. No.: I-183)
Anti-Mouse IL-1β-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-247)
Anti-Rat IL-1β-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-248)
Recombinant Mouse IL-1β (Leinco Prod. No.: I-205)
Recombinant Rat IL-1β (Leinco Prod. No.: I-212)
Anti-Mouse IL-1β (Antigen Aff. Pur.) (Leinco Prod. No.: I-162)
Anti-Mouse IL-1β Low Endotoxin Functional Formulation (Leinco Prod. No.: I-437)
Anti-Mouse IL-1β-Purified (Leinco Prod. No.: I-436)
Anti-Human IL-1β (Antigen Aff. Pur.) (Leinco Prod. No.: I-145)
Human Interleukin-1 Beta (IL-1β ) ELISA Development Kit (Leinco Prod. No.: I-333)
Anti-Rat IL-1β (Antigen Aff. Pur.) (Leinco Prod. No.: I-169)
Anti-Human IL-1β (Leinco Prod. No.: I-629)
Anti-Mouse IL-1β (Leinco Prod. No.: I-659)
Anti-Rat IL-1β (Leinco Prod. No.: I-672)
Anti-Human IL-1β (Leinco Prod. No.: I-171)
Anti-human IL-1β-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-757)
Anti-Mouse IL-1β-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-772)
Anti-Rat IL-1β-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-779)
Human IL-1β ELISA Development Kit (Leinco Prod. No.: I-817)
Rat IL-1β ELISA Development Kit (Leinco Prod. No.: I-387)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
