产品编号:I-272
产品描述:Anti-Human IL-17-Biotin
反应种属:human
实验方法:WB
标记:
规格:50ug
供应商:leinco
价格(RMB):5985.00
订购:
说明书:
Rabbit Anti-Human Interleukin-17 (IL-17)
Biotin
(CTLA8, IL17A)
Polyclonal Antibody
Prod. No.: I-272
Host: Rabbit
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Interleukin-17 (IL-17) recognizes Human IL-17. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 17 (IL-17) is a T cell cytokine that induces monocyte migration and inflammation.1 IL-17-producing cell could serve as a potential prognostic marker and a novel therapeutic target for hepatocellular carcinoma (HCC).2 IL-17 may be important in recruiting monocytes into the joints of patients with RA, supporting IL-17 as a therapeutic target in RA.1
Immunogen
Purified Recombinant Human IL-17A (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB
Western Blotting: To detect Human IL-17A this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-17A is 5 ng/lane under either reducing and 2 ng/lane under non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Pope RM et al. (2009) J Immunol. 182: 3884
2. Zheng L et al. (2009) J Hepatol.
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human IL-17 (Antigen Aff. Pur.) (Leinco Prod. No.: I-159)
Recombinant Human IL-17 (Leinco Prod. No.: I-198)
Recombinant Mouse IL-17 (Leinco Prod. No.: I-533)
Anti-Human IL-17 (Leinco Prod. No.: I-655)
Anti-Mouse IL-17 (Leinco Prod. No.: I-667)
Anti-Mouse IL-17 (Leinco Prod. No.: I-699)
Anti-Human IL-17-biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-770)
Anti-Mouse IL-17-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-775)
Recombinant Rat IL-17 (Leinco Prod. No.: I-812)
Human IL-17 ELISA Development Kit (Leinco Prod. No.: I-406)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
