产品编号:I-263
产品描述:Anti-Human IL-10-Biotin
反应种属:human
实验方法:WB, ELISA
标记:
规格:100ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Interleukin-10 (IL-10)
Biotin
(CSIF, IL10A, MGC126450, MGC126451, TGIF)
Polyclonal Antibody
Prod. No.: I-263
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Interleukin-10 (IL-10) recognizes Human IL-10. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is a homodimer protein produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and the Type 1 T helper cells. IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. It is released by cytotoxic T-cells to inhibit the actions of NK cells during the immune response to viral infection. IL-10 plays a key role in peripheral T cell tolerance to allergens, autoantigens, transplantation antigens and tumor antigens.1
Known Species Cross Reactivity: Hu+
Immunogen
Sf 21-Derived Recombinant Human Interleukin-10
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB,
Western Blotting: To detect Human IL-10 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-10 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1 - 0.4 µg/ml.
References
1. Akdis CA. et al. (2009) Mol. Immunol. 46: 622
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human IL-10 (Leinco Prod. No.: I-193)
Recombinant Mouse IL-10 (Leinco Prod. No.: I-211)
Recombinant Rat IL-10 (Cys 167 Tyr) (Leinco Prod. No.: I-222)
Anti-Human IL-10 (Antigen Aff. Pur.) (Leinco Prod. No.: I-154)
Anti-Mouse IL-10-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-264)
Anti-Rat IL-10-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-265)
Anti-Human IL-10 (Leinco Prod. No.: I-227)
Anti-Mouse IL-10 (Antigen Aff. Pur.) (Leinco Prod. No.: I-168)
Anti-Rat IL-10 (Antigen Aff. Pur.) (Leinco Prod. No.: I-243)
Recombinant Viral IL-10 (Leinco Prod. No.: I-596)
Anti-Human IL-10 (Leinco Prod. No.: I-635)
Anti-Porcine IL-10 (Leinco Prod. No.: I-696)
Anti-Canine IL-10 (Leinco Prod. No.: I-700)
Anti-Canine IL-10 (Leinco Prod. No.: I-701)
Anti-Viral IL-10 (EBV) (Leinco Prod. No.: I-718)
Anti-Viral IL-10 (EBV) (Antigen Aff. Pur.) (Leinco Prod. No.: I-749)
Human IL-10 ELISA Development Kit (Leinco Prod. No.: I-291)
Mouse IL-10 ELISA Development Kit (Leinco Prod. No.: I-292)
Recombinant Mouse IL-10 (Leinco Prod. No.: I-835)
Recombinant Human IL-10 (Leinco Prod. No.: I-836)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
