产品编号:I-249
产品描述:Anti-Human IL-3 Biotin
反应种属:human
实验方法:FC, WB, ICC
标记:
规格:100ug
供应商:leinco
价格(RMB):5595.00
订购:
说明书:
Goat Anti-Human Interleukin-3 (IL-3)
Biotin
(MCGF (Mast Cell Growth Factor), Multi-CSF, HCGF, P-Cell Stimulation Factor, MCGF, MGC79398, MGC79399, MULTI-CSF)
Polyclonal Antibody
Prod. No.: I-249
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Interleukin-3 (IL-3) recognizes Human IL-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 3 (colony-stimulating factor, multiple), also known as IL3 is a member of a family of growth factors, that supports the proliferation and development of hematopoietic precursors.1 IL3 can improve the body's natural response to disease as part of the immune system. It is secreted by activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human IL-3 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
FC, WB, ICC
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-3. A suggested method would be to stain cells expressing IL-3 with 3-10 µg per 1 x 106 cells in a total staining volume of 100 µl followed by UltraAvidin™- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104).
Western Blotting: To detect Human IL-3 this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-3 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Immunocytochemistry: 5-15 µg/ml
References
1. Laston D et al. (1998) Science 241: 1820
Related Products
UltraAvidin™- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104)
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse IL-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-250)
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Anti-Rat IL-3β (Antigen Aff. Pur.) (Leinco Prod. No.: I-343)
Anti-Mouse IL-3 (Antigen Aff. Pur.) (Leinco Prod. No.: I-163)
Anti-Human IL-3 (Antigen Aff. Pur.) (Leinco Prod. No.: I-147)
Human Interleukin-3 (IL-3) ELISA Development Kit (Leinco Prod. No.: I-285)
Recombinant Human IL-3 (Leinco Prod. No.: I-185)
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Mouse IL-3 ELISA Development Kit (Leinco Prod. No.: I-286)
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
