产品编号:I-241
产品描述:Anti-Human IGF-II-Biotin
反应种属:human
实验方法:WB
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Insulin Like Growth Factor-II (IGF-II)
Biotin
(Somatamedin A, Somatomedin C, C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974)
Polyclonal Antibody
Prod. No.: I-241
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Insulin Like Growth Factor-II (IGF-II) recognizes Human IGF-II. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Insulin-like growth factor 2 (IGF-2) is a polypeptide hormone with structural and functional homology with IGF-I and pro-insulin.1 IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor. The major role of IGF2 is growth promoting hormone during gestation. IGF-2 has been shown to be an important regulator of fetoplacental growth.2
Known Species Cross Reactivity: Hu+
Immunogen
E. coli-Derived Recombinant Human IGF-II
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB
Western Blotting: To detect Human IGF-II this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for rhIGF-II is approximately 5 ng/lane and 2 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. A. Hoeflich et al. (1994) Hormone Research 41: 66
2. Andrée Gruslin et al. (2005) The National Acad. of Sci.
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human IGF-II (Antigen Aff. Pur.) (Leinco Prod. No.: I-143)
Recombinant Mouse IGF-II (Leinco Prod. No.: I-586)
Anti-Human IGF-II (Leinco Prod. No.: I-650)
Anti-Mouse IGF-II (Leinco Prod. No.: I-710)
Recombinant Human IGF-II (Leinco Prod. No.: I-181)
Anti-Mouse IGF-II (Antigen Aff. Pur.) (Leinco Prod. No.: I-740)
Anti-Mouse IGF-II-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-800)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
