产品编号:I-277
产品描述:Anti-Human CXCL11-Biotin
反应种属:human
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Chemokine (C-X-C Motif) Ligand 11 (CXCL11)
Biotin
(H174, I-TAC, IP-9, IP9, MGC102770, SCYB11, SCYB9B, B-R1)
Polyclonal Antibody
Prod. No.: I-277
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Interferon-Inducible T-Cell Alpha Chemoattractant (I-TAC) recognizes Human I-TAC. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-X-C motif) ligand 11 (CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant (I-TAC) and Interferon-gamma-inducible protein 9 (IP-9). It is highly expressed in peripheral blood leukocytes, pancreas and liver, with moderate levels in thymus, spleen and lung and low expression levels were in small intestine, placenta and prostate.1 CXCL11 recruits activated Th1 cells to sites of inflammation2 and is thought to play a critical role in allograft rejection.3 CXCL11 may also be involved in multiple sclerosis (MS) pathogenesis.4
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human CXCL11 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Human I-TAC this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human I-TAC is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Neote K et al. (1998) J Exp Med. 187: 2009
2. Losey J et al. (2007) Eur Neurol. 58: 228
3. Barr ML et al. (2007) Mol Cell Biochem. 296: 1
4. Jacek Losy et al. (2007) Eur Neurol. 58: 228
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse I-TAC (Leinco Prod. No.: I-339)
Anti-Mouse CXCL11 (Leinco Prod. No.: C1443)
Anti-Human CXCL11(Antigen Aff. Pur.) (Leinco Prod. No.: I-238)
Anti-Mouse CXCL11 (Antigen Aff. Pur.) (Leinco Prod. No.: C1469)
Anti-Mouse CXCL11-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1513)
Recombinant Human CXCL11 (Leinco Prod. No.: I-228)
Anti-Human CXCL11 (Leinco Prod. No.: C1448)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
