产品编号:G142
产品描述:Anti-Human GM-CSF-Biotin
反应种属:human
实验方法:WB
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
Biotin
(CSF-2, MGI-1GM, Pluripoietin-Alpha)
Polyclonal Antibody
Prod. No.: G142
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) recognizes Human GM-CSF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Granulocyte-macrophage colony-stimulating factor, often abbreviated to GM-CSF is a pleiotropic cytokine that controls the production and function of blood cells.1 GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes.
GM-CSF has a critical role in regulation of surfactant homeostasis and alveolar macrophage innate immune functions in the lung.2
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human GM-CSF (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB
Western Blotting: To detect Human GM-CSF this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human GM-CSF is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human GM-CSF (Leinco Prod. No.: G124).
References
1. Micheal W. Parker et al. (2008) Cell 134: 496
2. Whitsett et al. (2002) Annual Review of Physiology 64: 775
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human GM-CSF (Leinco Prod. No.: G124)
Human GM-CSF ELISA Development Kit (Leinco Prod. No.: G149)
Anti-Mouse GM-CSF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G143)
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Recombinant Rat GM-CSF (Leinco Prod. No.: G596)
Anti-Human GM-CSF (Leinco Prod. No.: G665)
Anti-Mouse GM-CSF (Leinco Prod. No.: G670)
Anti-Rat GM-CSF (Leinco Prod. No.: G672)
Anti-Human GM-CSF (Leinco Prod. No.: G675)
Anti-Rat GM-CSF (Antigen Aff. Pur.) (Leinco Prod. No.: G681)
Anti-Rat GM-CSF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G691)
Mouse GM-CSF ELISA Development Kit (Leinco Prod. No.: G583)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
