产品编号:N127
产品描述:Anti-Human β-NGF-Biotin
反应种属:human
实验方法:
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human β-Nerve Growth Factor (β-NGF)
Biotin
(Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB)
Polyclonal Antibody
Prod. No.: N127
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human β-Nerve Growth Factor (β-NGF) recognizes Human β-NGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Nerve growth factor beta (NGF-beta) is a secreted protein that is important for the differentiation and survival of neurons and dermal keratinocytes.1 It is also involved in cell proliferation and survival, and it is a mediator of the immune response.2 NGF-beta accelerate ulcer healing.3
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human β-NGF (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB,
Western Blotting: To detect Human β-NGF this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human β-NGF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human β-NGF (Leinco Prod. No.: N116).
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) for the detection of Human β-NGF.
References
1. Schenck K et al. (2007) Eur J Oral Sci. 115: 344
2. Epplen JT et al. (2008) BMC Med Genet. 9: 107
3. Dai J et al. (2009) J Biomed Mater Res A
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human β-NGF (Leinco Prod. No.: N116)
Anti-Human β-NGF (Antigen Aff. Pur.) (Leinco Prod. No.: N111)
Recombinant Mouse β-NGF (Leinco Prod. No.: N180)
Human β-NGF ELISA Development Kit (Leinco Prod. No.: N146)
Anti-Human β-NGF (Leinco Prod. No.: N199)
Anti-Human β-NGF (Leinco Prod. No.: N145)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
