产品编号:I-233
产品描述:Anti-Rat IFN-γ
反应种属:mouse, rat
实验方法:ELISA, IHC, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5595.00
订购:
说明书:
Goat Anti-Rat Interferon-Gamma (IFN-γ)
Antigen Affinity Purified
(Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI)
Polyclonal Antibody
Prod. No.: I-233
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Rat/Mouse Interferon-Gamma (IFN-γ) recognizes Rat & Mouse IFN-γ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3
Known Species Cross Reactivity: Ms+, Rt+
Immunogen
Purified Recombinant Rat IFN-γ (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1.0 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Rat IFN-γ is approximately 5 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 0.5-5.0 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Rat IFN-γ bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat IFN-γ (at 2.5 ng/ml) is 0.08 - 0.2 µg/ml.
Western Blotting: To detect Rat IFN-γ this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Rat IFN-γ is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Rat IFN-γ (Leinco Prod. No.: I-223).
References
1. Goeddel DV et al. (1982) Nature 298: 859
2. Wilson CB et al. (2007) Adv. Immunol. 96: 41
3. Hume DA et al. (2004) J Leukoc Biol. 75: 163
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Rat IFN-γ (Leinco Prod. No.: I-223)
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Anti-Rat IFN-γ-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-203)
Anti-Human IFN-γ (Leinco Prod. No.: I-647)
Human IFN-γ ELISA Development Kit (Leinco Prod. No.: I-296)
Mouse IFN-γ ELISA Development Kit (Leinco Prod. No.: I-297)
Rat IFN-γ ELISA Development Kit (Leinco Prod. No.: I-381)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
