产品编号:T150

产品描述:Anti-Rat TNF-α

反应种属:mouse, rat

实验方法:ELISA, IHC-F, N, WB

标记:

规格:100ug

供应商:leinco

价格(RMB):5595.00

订购:

说明书:

Goat Anti-Rat Tumor Necrosis Factor-Alpha (TNF-α)
Antigen Affinity Purified
(Tumor Necrosis Factor Ligand Superfamily Member 2 (TNFSF2), Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin)
Polyclonal Antibody

Prod. No.: T150
Host:           Goat
Pkg. Size: 1 mg, 100 µg
Storage:      -20°C to -70°C

Description
Specificity:
Rabbit Anti-Rat Tumor Necrosis Factor-Alpha (TNF-α) recognizes Rat TNF-α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity.1 It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.2 TNF-α is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-α secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-α has been detected in synovial fluid of patients with rheumatoid arthritis.

Known Species Cross Reactivity: Ms+, Rt+
Immunogen
Purified Recombinant Rat TNF-α (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.01 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (Frozen), N, WB

References
1. El-Harith el-HA et al. (2004) Saudi Med J. 25: 135
2. Adolf GR et al. (1990) Infec Immun. 58: 3996

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.