产品编号:S512

产品描述:Anti-Rat SCF

反应种属:rat

实验方法:ELISA, ELISA, N, WB

标记:

规格:100ug

供应商:leinco

价格(RMB):6195.00

订购:

说明书:

Goat Anti-Rat Stem Cell Factor (SCF)
Antigen Affinity Purified
(KITLG, Steel Factor (SF), DKFZp686F2250, KL-1, Kitl, Mast Cell Growth Factor (MGF))
Polyclonal Antibody

Prod. No.: S512
Host:          Goat
Pkg. Size: 1 mg, 100 µg
Storage:       -20°C

Description
Specificity:
Goat Anti-Rat Stem Cell Factor (SCF) recognizes Rat SCF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Stem cell factor (SCF), otherwise known as KIT ligand or Steel factor1 is a paracrine regulator of germ cell development.2 SCF has the ability to stimulate the growth of hematopoietic progenitor cells and to generate colony-forming cells (CFC) from highly enriched populations of hematopoietic cells.3 SCF is produced by the Sertoli cells and involved in the local regulation of spermatogenesis4 and liver regeneration.5 SCF in conjunction with interleukin-31 may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung with the capacity to promote lung myofibroblast differentiation.6

Immunogen
Purified Recombinant Rat SCF (>98%)
Formulation
This antigen affinity purified polyclonal antibody is aseptically filtered and lyophilized from PBS with no carrier proteins or preservatives added.
Reconstitution
Aseptically reconstitute this antigen affinity purified polyclonal antibody using sterile deionized water to a concentration >0.2 mg/ml.
Storage and Stability
The lyophilized antibody should be stored desiccated at -20°C. The reconstituted antibody can be stored for at least six weeks at 4°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
ELISA Cap, Indirect ELISA, N, WB

References
1. Ian R et al. (2008) Culture of Animal Cells: A manual of basic technique
2. Toppari J et al. (1999) Endocrin 140: 1492
3. Adamson JW et al. (1991) PNAS 88: 7420
4. Hervé Lejeune et al. (2005) J of Endocrin. 186: 131
5. Ramadori G et al. (2009) Lab Invest.
6. Phan SH et al. (2009) AM J Pathol. 174: 390
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.