产品编号:R137
产品描述:Anti-Rat RANTES
反应种属:rat
实验方法:ELISA, ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5520.00
说明书:
Goat Anti-Rat Regulated upon Activation Normal T Cell Express Sequence (RANTES)
Antigen Affinity Purified
(CCL5, D17S136E, MGC17164, SCYA5, SIS-Delta, TCP228)
Polyclonal Antibody
Prod. No.: R137
Host: Goat
Pkg. Size: 1 mg, 50 µg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Rat Regulated upon Activation Normal T Cell Express Sequence (RANTES) recognizes Rat RANTES. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
RANTES also known as Chemokine (C-C motif) ligand 5 (CCL5), a CC-chemokine1 is an important mediators of the immune and inflammatory response.2 It is also a potent chemoattractant for T cells 3 and eosinophils.4 RANTES is involved in many inflammatory diseases, for example, bronchial asthma, delayed-type hypersensitivity reactions, viral infections, arthritis, chronic eosinophilic pneumonia, and idiopathic interstitial pneumonia.5
Immunogen
Purified Recombinant Rat RANTES (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Rat RANTES is approximately 1 ng/well.
Neutralization: This antibody is useful for neutralization of Rat RANTES bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat RANTES (at 0.2 ng/ml) is 1 - 4 µg/ml.
Western Blotting: To detect Rat RANTES this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Rat RANTES is 10 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Lee SC et al. (2004) J Neurochem. 90: 297
2. Borkowski J et al. (2004) Arch Immunol Ther Exp (Warsz) 52: 201
3. Geiger H et al. (2005) Kidney Blood Press Res 28: 48
4. Hirata I. et al. (2008) Dig Dis Sci.
5. Emilie D et al. (1994) J Exp Med. 179: 1689
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.