产品编号:I-343
产品描述:Anti-Rat IL-3β
反应种属:rat
实验方法:ELISA, ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5250.00
订购:
说明书:
Goat Anti-Rat Interleukin-3β (IL-3)
Antigen Affinity Purified
(MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor )
Polyclonal Antibody
Prod. No.: I-343
Host: Goat
Pkg. Size: 1 mg, 50 µg, 100 µg
Storage: -20°C
Description
Specificity:
Goat Anti-Rat Interleukin-3β (IL-3) recognizes Rat IL-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Rat IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.
Immunogen
Purified Recombinant Rat IL-3 (>98%)
Formulation
This antigen affinity purified polyclonal antibody is aseptically filtered and lyophilized from PBS with no carrier proteins or preservatives added.
Reconstitution
Aseptically reconstitute this antigen affinity purified polyclonal antibody using sterile deionized water to a concentration >0.2 mg/ml.
Storage and Stability
The lyophilized antibody should be stored desiccated at -20°C. The reconstituted antibody can be stored for at least six weeks at 4°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
ELISA Cap, Indirect ELISA, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 µg/ml. A suitable detection antibody is Anti-Rat IL-3β-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-344) at a concentration of approximately 0.25–1.0 µg/ml. When used in conjunction with an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" (Leinco Prod. No.: T118), the sensitivity of this sandwich ELISA is 0.2–0.4 ng/well. A suggested standard for this assay is Recombinant Rat IL-3 (Leinco Prod. No.: I-281).
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-2.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Rat IL-3 is approximately 0.2-0.4 ng/well.
Neutralization: This antibody is useful for neutralization of Rat IL-3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat IL-3 (at 100 ng/ml) is 2.7-4.0 µg/ml.
Western Blotting: To detect Rat IL-3 by Western blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible second step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343), the detection limit for Rat IL-3 is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. A suitable Western blotting control is Recombinant Rat IL-3 (Leinco Prod. No.: I-281).
Related Products
Anti-Rat IL-3β-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-344)
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" (Leinco Prod. No.: T118)
Recombinant Rat IL-3 (Leinco Prod. No.: I-281)
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human IL-3 Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-249)
Anti-Mouse IL-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-250)
Anti-Mouse IL-3 (Antigen Aff. Pur.) (Leinco Prod. No.: I-163)
Anti-Human IL-3 (Antigen Aff. Pur.) (Leinco Prod. No.: I-147)
Human Interleukin-3 (IL-3) ELISA Development Kit (Leinco Prod. No.: I-285)
Recombinant Human IL-3 (Leinco Prod. No.: I-185)
Anti-Human IL-3 (Leinco Prod. No.: I-630)
Anti-Mouse IL-3 (Leinco Prod. No.: I-661)
Anti-Human IL-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-758)
Anti-Human IL-3 (Leinco Prod. No.: I-686)
Mouse IL-3 ELISA Development Kit (Leinco Prod. No.: I-286)
Recombinant Mouse IL-3 (Leinco Prod. No.: I-206)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
