产品编号:V104

产品描述:Anti-Mouse VEGF

反应种属:mouse

实验方法:ELISA, IHC-F, N, WB

标记:

规格:100ug

供应商:leinco

价格(RMB):5595.00

订购:

说明书:

Goat Anti-Mouse Vascular Endothelial Growth Factor (VEGF)
Antigen Affinity Purified
(VEGFA, MGC70609, VPF, VAS, Folliculostellate Cell-Derived Growth Factor, Glioma-Derived Endothelial Cell Mitogen)
Polyclonal Antibody

Prod. No.: V104
Host:         Goat
Pkg. Size: 1 mg, 100 µg
Storage:      -20°C to -70°C

Description
Specificity:
Rabbit Anti-Mouse Vascular Endothelial Growth Factor (VEGF) recognizes Mouse VEGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3,4,5,6

Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse VEGF (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 0.5 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.2 mg/ml.
Endotoxin
<0.04 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, IHC (Frozen), N, WB

References
1. Folkman J et al. (2008) FASEB J. 22: 3728
2. Goodsell DS et al. (2002) The Oncologist 7: 569
3. Mugishima H. et al. (2006) J Atheroscler Thromb. 13: 130
4. Claesson-Welsh L et al. (2006) Exp Cell Res. 312: 549
5. Claesson-Welsh L et al. (1999) Trends Biochem Sci. 28: 488
6. Ellis LM et al. (2005) J Clin Oncol. 23: 1011
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.