产品编号:T149
产品描述:Anti-Mouse TNF-α
反应种属:mouse
实验方法:ELISA, IHC, FC, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5595.00
订购:
说明书:
Goat Anti-Mouse Tumor Necrosis Factor-Alpha (TNF-α)
Antigen Affinity Purified
(Tumor Necrosis Factor Ligand Superfamily Member 2 (TNFSF2), Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin)
Polyclonal Antibody
Prod. No.: T149
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Tumor Necrosis Factor-Alpha (TNF-α) recognizes Mouse TNF-α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity.1 It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.2 TNF-α is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-α secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-α has been detected in synovial fluid of patients with rheumatoid arthritis.
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse TNF-α (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 0.5 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.2 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months
Reported Applications
ELISA Cap, IHC (NBF/Par.), FC, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/ml. A suitable detection antibody is Anti-Mouse TNF-α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T188). A suggested standard for this assay is Recombinant Mouse TNF-α (Leinco Prod. No.: T156).
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Mouse TNF-α bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse TNF-α (at 0.25 ng/ml) is 0.1-0.4 µg/ml.
Western Blotting: To detect Mouse TNF-α this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse TNF-α is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. El-Harith el-HA et al. (2004) Saudi Med J. 25: 135
2. Adolf GR et al. (1990) Infec Immun. 58: 3996
Related Products
Anti-Mouse TNF-α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T188)
Recombinant Mouse TNF-α (Leinco Prod. No.: T156)
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human TNF-α (Leinco Prod. No.: T153)
Anti-Mouse TNF-α - Low Endotoxin Functional Formulation (Leinco Prod. No.: T258)
Anti-Mouse TNFα-Purified (Leinco Prod. No.: T259)
Anti-Mouse TNFα-R-phycoerythrin (Leinco Prod. No.: T262)
Human TNF-α ELISA Development Kit (Leinco Prod. No.: T200)
Recombinant Mouse TNF-α (Leinco Prod. No.: T376)
Anti-Human TNF-α (Leinco Prod. No.: T427)
Anti-Rat TNF-α (Leinco Prod. No.: T442)
Anti-Porcine TNF-α (Leinco Prod. No.: T455)
Anti-Porcine TNF-α (Leinco Prod. No.: T456)
Anti-Human TNF-α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T187)
Anti-Human TNF-α (Antigen Aff. Pur.) (Leinco Prod. No.: T146)
Anti-Human TNF-α (Monoclonal Antibody) (Leinco Prod. No.: T190)
Anti-Rat TNF-α (Antigen Aff. Pur.) (Leinco Prod. No.: T150)
Anti-Rat TNF-α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T189)
Recombinant Rat TNF-α (Leinco Prod. No.: T157)
Anti-Human Extracellular TNF-α-Fluorescein (FITC) (Leinco Prod. No.: T505)
Rat TNF-α ELISA Development Kit (Leinco Prod. No.: T518)
Mouse TNF-α ELISA Development Kit (Leinco Prod. No.: T519)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
