产品编号:R1144

产品描述:Anti-Mouse RANKL

反应种属:mouse

实验方法:ELISA, IHC-F, N, WB

标记:

规格:100ug

供应商:leinco

价格(RMB):5325.00

订购:

说明书:

Goat Anti-Mouse Receptor Activator of NFκB Ligand (RANKL)
Antigen Affinity Purified
(CD254, TRANCE, hRANKL2, sOdf, TNF-Related Activation-Induced Cytokine [TRANCE], Osteoprotegrin Ligand [OPGL], Osteoclast Differentiation Factor [ODF], TNFSF11)
Polyclonal Antibody

Prod. No.: R1144
Host:          Goat
Pkg. Size: 1 mg, 100 µg
Storage:      -20°C to -70°C

Description
Specificity:
Rabbit Anti-Mouse soluble Receptor Activator of NFkB Ligand (RANKL) recognizes Mouse RANKL. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
RANKL (Receptor Activator for Nuclear Factor κ B Ligand), also known as TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and ODF (osteoclast differentiation factor), is a member of the tumor necrosis factor family cytokine.1 RANKL is essential for osteoclast formation, activation, and survival.2 RANKL through interaction with its receptor RANK can modulate matrix degradation and inflammation.3 RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy. Consequently, novel drugs specifically targeting RANK, RANKL, and their signaling pathways in osteoclasts are expected to revolutionize the treatment of various ailments associated with bone loss, such as arthritis, periodontal disease, cancer metastases, and osteoporosis.4

Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse RANKL (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 100 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (Frozen), N, WB

References
1. H Takayanagi et al. (2003) Arthritis Res Ther. 5: 20
2. Kostenuik PJ et al. (2005) Endocrinology 146: 3235
3. Holven KB et al. (2009) Stroke 40: 241
4. Penninger JM et al. (2008) Ann N Y Acad Sci. 1143: 123
Related Products
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Recombinant Mouse RANK Ligand (Leinco Prod. No.: R1134)
Anti-Human RANKL (Leinco Prod. No.: R1242)
RANKL Blocking Peptide (Leinco Prod. No.: R1243)
Anti-Human RANKL (Leinco Prod. No.: T448)
Recombinant Mouse RANK Ligand (Leinco Prod. No.: R1255)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.