产品编号:M177
产品描述:Anti-Mouse MIP-1α
反应种属:mouse
实验方法:ELISA, IHC, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Macrophage Inflammatory Protein 1 Alpha (MIP-1α)
Antigen Affinity Purified
(G0S19-1, LD78Alpha, MIP1A, SCYA3, CCL3)
Polyclonal Antibody
Prod. No.: M177
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Macrophage Inflammatory Protein 1 Alpha (MIP-1α) recognizes Mouse MIP-1α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Macrophage inflammatory protein 1 alpha (MIP-1 alpha) is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.1 MIP-1 alpha is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.2 The role of MIP-1 alpha seems to be related to production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.3
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse MIP-1α (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115). The detection sensitivity of this indirect ELISA for Mouse MIP-1α is approximately 0.4 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Mouse MIP-1α bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse MIP-1α (at 10 ng/ml) is 0.05-0.2 µg/ml.
Western Blotting: To detect Mouse MIP-1α this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse MIP-1α is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. IB Pragnell et al. (1994) J. Biol. Chem. 269: 4974
2. DN Cook et al. (1996) J. of Leukocyte Biol. 59: 61
3. PA Ward et al. (1995) J of Immunol. 154: 4793
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
