产品编号:M1007

产品描述:Anti-Mouse MCP-2

反应种属:mouse

实验方法:ELISA, ELISA, N, WB

标记:

规格:100ug

供应商:leinco

价格(RMB):5925.00

订购:

说明书:

Rabbit Anti-Mouse Monocyte Chemotactic Protein-2 (MCP-2)
Antigen Affinity Purified
(SCYA8, CCL8, HC14, SCYA10, Small Inducible Cytokine A8, Chemokine (C-C Motif) Ligand 8, Monocyte Chemotactic, C-C Motif Chemokine 8)
Polyclonal Antibody

Prod. No.: M1007
Host:          Rabbit
Pkg. Size: 1 mg, 100 µg
Storage:        -20°C

Description
Specificity:
Rabbit Anti-Mouse Monocyte Chemotactic Protein-2 (MCP-2) recognizes Mouse MCP-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Monocyte chemotactic protein-2 (MCP-2) is a non-glycosylated polypeptide that is a member of the C-C chemokine subfamily and it specifically attracts monocytes, lymphocytes, basophils and eosinophils. It also plays a role in neoplasia and inflammatory host responses. MCP-2 can bind heparin.1

Immunogen
Purified Recombinant Mouse MCP-2 (>98%)
Formulation
This antigen affinity purified polyclonal antibody is aseptically filtered and lyophilized from PBS with no carrier proteins or preservatives added.
Reconstitution
Aseptically reconstitute this antigen affinity purified polyclonal antibody using sterile deionized water to a concentration >0.2 mg/ml.
Storage and Stability
The lyophilized antibody should be stored desiccated at -20°C. The reconstituted antibody can be stored for at least six weeks at 4°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
ELISA Cap, Indirect ELISA, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5 - 1.5 µg/ml. The sensitivity of this sandwich ELISA is 0.2 ng/well.
Western Blotting: To detect Mouse MCP-2 this polyclonal antibody can be used at a concentration of 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse MCP-2 is 3 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

References
1. van Damme J. et al. (1998) J. Immunol. 160: 4034
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse MCP-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M1008)
Anti-Human MCP-2 (Antigen Aff. Pur.) (Leinco Prod. No.: M143)
Anti-Human MCP-2 (Leinco Prod. No.: M1090)
Anti-Human MCP-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M124)
Recombinant Human MCP-2 (Leinco Prod. No.: M157)
Recombinant Mouse MCP-2 (Leinco Prod. No.: M1099)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.