产品编号:M1101
产品描述:Anti-Mouse M-CSF
反应种属:mouse
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Macrophage Colony Stimulating Factor (M-CSF)
Antigen Affinity Purified
(CSF-1, MGI-IM, MGC31930)
Polyclonal Antibody
Prod. No.: M1101
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Macrophage Colony Stimulating Factor (M-CSF) recognizes Mouse M-CSF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Macrophage colony-stimulating factor, or M-CSF, is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis, and secondary cytokine production in monocytes and macrophages.1 It binds to the Colony stimulating factor 1 receptor. M-CSF is known as one of the factors essential for osteoclast development.2
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse M-CSF (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Mouse M-CSF is approximately 0.16 ng/well.
Neutralization: This antibody is useful for neutralization of Mouse M-CSF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse M-CSF (at 2.5 ng/ml) is 0.015 - 0.035 µg/ml.
Western Blotting: To detect Mouse M-CSF this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse M-CSF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse M-CSF (Leinco Prod. No.: M1098).
References
1. Nemunaitis J. et al. (1998) Clin. Infect. Dis. 26: 1279
2. Ko JS. et al. (2002) Exp Mol Med 34: 340
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse M-CSF (Leinco Prod. No.: M1098)
Anti-Human M-CSF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M140)
Recombinant Rat M-CSF (Leinco Prod. No.: M1227)
Anti-Human M-CSF (Antigen Aff. Pur.) (Leinco Prod. No.: M141)
Anti-Human M-CSF (Leinco Prod. No.: M1269)
Anti-Mouse M-CSF (Leinco Prod. No.: M1276)
Anti-Mouse M-CSF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M1102)
Mouse M-CSF ELISA Development Kit (Leinco Prod. No.: M1324)
Recombinant Human M-CSF (Leinco Prod. No.: M155)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
