产品编号:L115
产品描述:Anti-Mouse Leptin
反应种属:mouse
实验方法:ELISA, IHC-F, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Leptin
Antigen Affinity Purified
(Obesity Protein (OB), B219, LEP, OBS)
Polyclonal Antibody
Prod. No.: L115
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Mouse Leptin recognizes Mouse Leptin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Leptin is a neuroendocrine hormone secreted from adipose tissue that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. Leptin is one of the most important adipose derived hormones.1 It interacts with six types of receptors and once it binds to OB-Receptor it activates the stat3, which is phosphorylated and travels to the nucleus to effect changes in gene expression. One of the main effects on gene expression is the down-regulation of the expression of endocannabinoids.2 High leptin levels are associated with psoriasis.3
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse Leptin (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, IHC (Frozen), N, WB
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 5-15 µg/ml.
Neutralization: This antibody is useful for neutralization of Mouse Leptin bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse Leptin (at 2.5 ng/ml) is 0.05-0.3 µg/ml.
Western Blotting: To detect Mouse Leptin this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse Leptin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Mantzoros CS et al.(2006) Nat Clin Pract Endocrinol Metab. 2: 318
2. Hill RA. et al.(2002) Int. J. Obes. Relat. Metab. Disord. 26: 1407
3. Chen Y-J. et al.(2008) Arch Derm. 144: 1571
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human Leptin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L110)
Recombinant Equine Leptin (Leinco Prod. No.: L179)
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Anti-Rat Leptin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L155)
Anti-Human Leptin (Leinco Prod. No.: L109)
Anti-Mouse Leptin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L111)
Recombinant Bovine Leptin (Leinco Prod. No.: L178)
Recombinant Chicken Leptin (Leinco Prod. No.: L181)
Recombinant Porcine Leptin (Leinco Prod. No.: L180)
Recombinant Rat Leptin (Leinco Prod. No.: L146)
Recombinant Human Leptin (Leinco Prod. No.: L116)
Human Leptin ELISA Development Kit (Leinco Prod. No.: L234)
Mouse Leptin ELISA Development Kit (Leinco Prod. No.: L236)
Recombinant Mouse Leptin (Leinco Prod. No.: L117)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
