产品编号:J101
产品描述:Anti-Mouse MCP-1
反应种属:mouse
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Monocyte Chemotactic Protein (MCP-1)
Antigen Affinity Purified
(CCL2, GDCF-2, GDCF-2 HC11, HC11, HSMCR30, MCAF, MGC9434, SCYA2, SMC-CF, JE)
Polyclonal Antibody
Prod. No.: J101
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Mouse Monocyte Chemotactic Protein (JE/MCP-1) recognizes Mouse JE/MCP-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Monocyte chemotactic protein-1 (MCP-1) is an essential chemokine produced predominantly by macrophages and endothelial cells and is a potent chemotactic factor for monocytes.1,2,3 It can induce the proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer). MCP-1 is also a potent activator of human basophils, induces degranulation and the release of histamines. MCP-1 is involved in the pathogenesis of atherosclerosis and myocardial infarction.4
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse JE/MCP-1 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB
Western Blotting: To detect Mouse MCP-1 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse MCP-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse MCP-1 (Leinco Prod. No.: J100).
References
1. Yoshimura T. et al. (1989) J. Exp. Med. 169: 1449
2. Oppenheim J.J. et al. (1989) J. Exp. Med. 169: 1485
3. Rollins B.J. et al. (1997) Blood 90: 909
4. Benjamin EJ. et al. (2005) Ciculation 112: 1113
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse MCP-1 (Leinco Prod. No.: J100)
Anti-Rat MCP-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M123)
Anti-Human MCP-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M122)
Anti-Rat MCP-1 (Antigen Aff. Pur.) (Leinco Prod. No.: M150)
Anti-Human MCP-1 (Antigen Aff. Pur.) (Leinco Prod. No.: M142)
Anti-Human MCP-1 (Leinco Prod. No.: M1271)
Anti-Human MCP-1 (Leinco Prod. No.: M152)
Anti-Mouse MCP-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: J102)
Recombinant Rat MCP-1 (Leinco Prod. No.: M164)
Mouse MCP-1 ELISA Development Kit (Leinco Prod. No.: J104)
Human MCP-1 ELISA Developmant Kit (Leinco Prod. No.: M1109)
Recombinant Human MCP-1 (Mucin) (Leinco Prod. No.: M1256)
Recombinant Human MCP-1 (Leinco Prod. No.: M156)
Rat MCP-1 ELISA Development Kit (Leinco Prod. No.: M1323)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
