产品编号:I-503

产品描述:Anti-Mouse IL-31(NT)

反应种属:mouse

实验方法:IHC, WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5700.00

订购:

说明书:

Rabbit Anti-Mouse Interleukin-31 (IL-31)
Purified No Carrier Protein
Polyclonal Antibody

Prod. No.: I-503
Host:         Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:      2-8°C

Description
Specificity:
Rabbit Anti-Mouse Interleukin-31 (IL-31) recognizes an epitope near the N-terminus of Mouse IL-31. This polyclonal antibody was purified using affinity chromatography.

Background:
Interleukin-31 (IL-31) is a T-cell cytokine, which is preferentially produced by type 2 helper T cells. The structure of IL-31 places it in the IL-6 family of cytokines. IL-31 signals via a receptor complex that is composed of IL-31 receptor A and oncostatin M receptor subunits. IL-31 is believed to play a role in inflammation of the skin.1 IL-31 represents a novel target for antipruritic drug development.2

Known Species Cross Reactivity: Ms+
Immunogen
IL-31 (NT) Blocking Peptide (Leinco Prod. No.: I-515)
Blocking Peptide
IL-31 (NT) Blocking Peptide (Leinco Prod. No.: I-515)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Mouse IL-31 this polyclonal antibody can be used at a concentration of 2.5 - 5.0 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is RAW 264.7 Whole Cell Lysate (Leinco Prod. No.: R1174).

References
1. Stacey R Dillon et al.(2004) Nature Immunol. 5: 752
2. Homey B. et al.(2006) J Allergy Clin Immunol. 117: 411

Related Products
IL-31 (NT) Blocking Peptide (Leinco Prod. No.: I-515)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
RAW 264.7 Whole Cell Lysate (Leinco Prod. No.: R1174)
Anti-Human IL-31-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-420)
Recombinant Human IL-31 (Leinco Prod. No.: I-417)
Recombinant Mouse IL-31 (Leinco Prod. No.: I-418)
Anti-Human IL-31 (Intermediate Domain) (Leinco Prod. No.: I-502)
IL-31 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: I-514)
Anti-Human IL-31 (Antigen Aff. Pur.) (Leinco Prod. No.: I-419)
Human IL-31 ELISA Development Kit (Leinco Prod. No.: I-830)
Recombinant Human Interleukin-31 (Leinco Prod. No.: I-839)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.