产品编号:C1420
产品描述:Anti-Mouse CXCL1
反应种属:mouse
实验方法:ELISA, N, WB
标记:
规格:500ug
供应商:leinco
价格(RMB):4875.00
订购:
说明书:
Anti-Mouse CXCL1
Purified No Carrier Protein
(GRO1, GROa, MGSA, MGSA Alpha, NAP-3, SCYB1)
Monoclonal Antibody
Prod. No.: C1420
Clone: 48415
Isotype: Rat IgG2a
Pkg. Size: 1 mg, 500 µg
Storage: -20°C to -70°C
Description
Specificity:
Rat Anti-Mouse CXCL1 (Clone 48415) recognizes an epitope on Mouse CXCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).1 CXCL1 is expressed by macrophages, neutrophils and epithelial cells.2 CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.3,4
Host Species
Rat
Immunogen
Purified Recombinant Mouse CXCL1 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is Anti-Mouse CXCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: K124) at a concentration of approximately 0.1 - 0.4 µg/ml. A suggested standard for this assay is PN:K144.
Neutralization: This antibody is useful for neutralization of Mouse CXCL1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CXCL1 (at 30 ng/ml) is 0.05 - 0.25 µg/ml.
Western Blotting: To detect Mouse CXCL1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse CXCL1 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Sager R et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732
2. Grotendorst GR et al. (1990) Mol. Cell. Biol. 10: 5596
3. Miller RH et al. (2002) Cell 110: 373
4. Richmond A et al. (2000) J. Invest. Dermatol. 115: 234
Related Products
Anti-Mouse CXCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: K124)
PN:K144
Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CXCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G145)
Anti-Mouse CXCL1 (Antigen Aff. Pur.) (Leinco Prod. No.: K125)
Anti-Rat CXCL1 (Antigen Aff. Pur.) (Leinco Prod. No.: G121)
Anti-Rat CXCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G144)
Anti-Mouse KC-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: K133)
Recombinant Mouse CXCL1 (aa 29 - 96) Truncated Form (Leinco Prod. No.: K134)
Anti-Human CXCL1 (Leinco Prod. No.: C1393)
Anti-Mouse CXCL1 (Leinco Prod. No.: K135)
Anti-Human CXCL1 (Leinco Prod. No.: C1394)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
