产品编号:I-364
产品描述:Anti-Mouse IL-18BP
反应种属:mouse
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Interleukin-18 Binding Protein (IL-18BP)
Antigen Affinity Purified
Polyclonal Antibody
Prod. No.: I-364
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Interleukin-18 Binding Protein (IL-18BP) recognizes Mouse IL-18BP. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Mouse IL-18BP is a circulating glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 with high affinity and neutralizing its biological activity. IL-18BP has no amino acid sequence homology to IL-18 or IL-1 receptors. The gene for human IL-18BP encodes at least four isoforms by alternative splicing. The IL-18BP isoforms A and C each contain one immunoglobulin (Ig)-like C2-type domain while isoforms B and D lack the complete Ig domain. The complete Ig domain has been shown to be essential for IL-18BPs binding and neutralizing properties. Several poxviruses have been shown to encode proteins with sequence similarity to human and mouse IL-18BPs that also bind and inhibit IL-18 bioactivities. This could lead to viral modulation of host immune responses.
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant IL-18BP (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<10 EU/ µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Mouse IL-18BP is approximately 0.3 ng/well.
Neutralization: This antibody is useful for neutralization of Mouse IL-18BP bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IL-18BP (at 1 ng/ml) is 10 - 30 µg/ml.
Western Blotting: To detect Mouse IL-18BP this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse IL-18BP is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse IL-18BPc-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-365)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
