产品编号:I-348
产品描述:Anti-Mouse IL-13
反应种属:mouse
实验方法:ELISA, N, WB, ICC
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Interleukin-13 (IL-13)
Antigen Affinity Purified
(ALRH, BHR1, MGC116786, P600, NC30)
Polyclonal Antibody
Prod. No.: I-348
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Mouse Interleukin-13 (IL-13) recognizes Mouse IL-13. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 13 (IL-13) is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells1, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα), which is also a component of the IL-4 receptor, and at least one of two known IL-13-specific binding chains.1 The functions of IL-13 overlap considerably with those of IL-4. Although IL-13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse IL-13 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.01 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (Frozen), N, WB, ICC
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Mouse IL-13 is approximately 1.5 ng/well.
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 5 - 15 µg/ml.
Neutralization: This antibody is useful for neutralization of Mouse IL-13 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IL-13 (at 10 ng/ml) is 0.05 - 0.15 µg/ml.
Western Blotting: To detect Mouse IL-13 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse IL-13 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse IL-13 (Leinco Prod. No.: I-278).
Immunocytochemistry: This antibody will detect IL-13 in cells. The working dilution is 5 - 15 μg/ml.
References
1. Wynn TA. et al. (2003) Annu Rev Immunol. 21: 425
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse IL-13 (Leinco Prod. No.: I-278)
Anti-Human IL-13-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-269)
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Anti-Mouse IL-13-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-349)
Anti-Human IL-13 (Leinco Prod. No.: I-634)
Anti-Mouse IL-13 (Leinco Prod. No.: I-665)
Recombinant Rat IL-13 (Leinco Prod. No.: I-220)
Mouse IL-13 ELISA Development Kit (Leinco Prod. No.: I-403)
Recombinant Human IL-13 (Leinco Prod. No.: I-195)
Anti-Rat IL-13 (Antigen Aff. Pur.) (Leinco Prod. No.: I-854)
Anti-Rat IL-13-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-864)
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
