产品编号:I-166
产品描述:Anti-Mouse IL-7
反应种属:mouse
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Interleukin-7 (IL-7)
Antigen Affinity Purified
(Lymphopoietin 1, LP-1, pre-B Cell Factor)
Polyclonal Antibody
Prod. No.: I-166
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Mouse Interleukin-7 (IL-7) recognizes Mouse IL-7. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 7 (IL-7) is a glycoprotein cytokine initially referred to as pre-B cell growth factor because of its growth-promoting activity for cells early in the B lymphocyte lineage. Subsequent to IL-7 gene cloning and expression, IL-7 was also found to influence the activity of cells in the T lineage.1 IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells. It also stimulates proliferation of all cells in the lymphoid lineage (B and T lymphocytes and NK cells). IL-7 is not only a potent grown stimulus for pre-B cells, but also plays a role in T cell activation.2 IL-7 has significant clinical and biomedical potential, such as cancer therapy and HIV infections.3
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse IL-7 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.8 µg/ml. A suitable detection antibody is Anti-Mouse IL-7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-259) at a concentration of approximately 0.2 0.8 µg/ml. A suggested standard for this assay is Recombinant Mouse IL-17 (Leinco Prod. No.: I-533).
Neutralization: This antibody is useful for neutralization of Mouse IL-7 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IL-7 (at 1.5 ng/ml) is 0.2 - 0.4 µg/ml.
Western Blotting: To detect Mouse IL-7 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse IL-7 is 5.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse IL-17 (Leinco Prod. No.: I-533).
References
1. Namen AE. et al. (1990) Int J Cell Cloning 1: 168
2. Reed SG. et al. (1989) J Exp Med. 169: 707
3. Prakash S. et al. (2008) Appl. of Biochem Biotechnol. 151: 93
Related Products
Anti-Mouse IL-7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-259)
Recombinant Mouse IL-17 (Leinco Prod. No.: I-533)
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human IL-7 (Leinco Prod. No.: I-189)
Anti-Human IL-7 (Antigen Aff. Pur.) (Leinco Prod. No.: I-151)
Recombinant Rat IL-7 (Leinco Prod. No.: I-192)
Anti-Human IL-7 (Leinco Prod. No.: I-175)
Anti-Human IL-7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-258)
Recombinant Mouse IL-7 (Leinco Prod. No.: I-209)
Human IL-7 ELISA Development Kit (Leinco Prod. No.: I-826)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
