产品编号:I-163
产品描述:Anti-Mouse IL-3
反应种属:mouse
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5595.00
订购:
说明书:
Goat Anti-Mouse Interleukin-3 (IL-3)
Antigen Affinity Purified
(MCGF (Mast Cell Growth Factor), Multi-CSF, HCGF, P-Cell Stimulation Factor, MCGF, MGC79398, MGC79399, MULTI-CSF)
Polyclonal Antibody
Prod. No.: I-163
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Mouse Interleukin-3 (IL-3) recognizes Mouse IL-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Interleukin 3 (colony-stimulating factor, multiple), also known as IL3 is a member of a family of growth factors, that supports the proliferation and development of hematopoietic precursors.1 IL3 can improve the body's natural response to disease as part of the immune system. It is secreted by activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse IL-3 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<10 ng/mg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Mouse IL-3 is approximately 0.16 ng/well.
Western Blotting: To detect Mouse IL-3 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse IL-3 is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse IL-3 (Leinco Prod. No.: I-206).
References
1. Laston D et al. (1998) Science 241: 1820
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse IL-3 (Leinco Prod. No.: I-206)
Anti-Human IL-3 Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-249)
Anti-Mouse IL-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-250)
Recombinant Rat IL-3 (Leinco Prod. No.: I-281)
Anti-Rat IL-3β (Antigen Aff. Pur.) (Leinco Prod. No.: I-343)
Anti-Human IL-3 (Antigen Aff. Pur.) (Leinco Prod. No.: I-147)
Human Interleukin-3 (IL-3) ELISA Development Kit (Leinco Prod. No.: I-285)
Recombinant Human IL-3 (Leinco Prod. No.: I-185)
Anti-Human IL-3 (Leinco Prod. No.: I-630)
Anti-Mouse IL-3 (Leinco Prod. No.: I-661)
Anti-Human IL-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-758)
Anti-Human IL-3 (Leinco Prod. No.: I-686)
Mouse IL-3 ELISA Development Kit (Leinco Prod. No.: I-286)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
