产品编号:I-340
产品描述:Anti-Mouse IGF-I
反应种属:mouse
实验方法:ELISA, IHC-F, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Insulin Like Growth Factor-I (IGF-1)
Antigen Affinity Purified
(Somatamedin C, IGF-IA )
Polyclonal Antibody
Prod. No.: I-340
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Insulin Like Growth Factor-I (IGF-1) recognizes Mouse IGF-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Production of IGF-1is stimulated by growth hormone. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. In addition to the insulin-like effects, IGF-1 can also regulate cell growth and development, especially in nerve cells, as well as cellular DNA synthesis. IGF-1 is closely related to a second protein called IGF-2. IGF-1 is used by physicians as a screening test for growth hormone deficiency and excess. The IGF signaling pathway appears to play a crucial role in cancer. Several studies have shown that increased levels of IGF lead to an increased risk of cancer.1
Known Species Cross Reactivity: Ms+
Immunogen
E. coli-Derived Recombinant Mouse IGF-I
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Mouse IGF-1 is approximately 1.0 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Mouse IGF-1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IGF-1 (at 15 ng/ml) is 0.2 – 1.0 µg/ml.
Western Blotting: To detect Mouse IGF-1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse IGF-1 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Govindan R et al. (2006) Journal of thoracic oncology 1: 607
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human IGF-I-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-235)
PN:
Recombinant Human IGF-I (Leinco Prod. No.: I-180)
Anti-Human IGF-I (Antigen Aff. Pur.) (Leinco Prod. No.: I-142)
Recombinant Mouse IGF-I (Leinco Prod. No.: I-239)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
