产品编号:F109
产品描述:Anti-Mouse FGF-9
反应种属:mouse
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5775.00
订购:
说明书:
Rabbit Anti-Mouse Fibroblast Growth Factor (FGF-9)
Antigen Affinity Purified
(Growth Factor-9, GAF (Glia-Activating Factor), HBGF-9, HBFG-9, MGC119914, MGC119915)
Polyclonal Antibody
Prod. No.: F109
Host: Rabbit
Pkg. Size: 1 mg, 100 µg
Storage: -20°C
Description
Specificity:
Rabbit Anti-Mouse Fibroblast Growth Factor (FGF-9) recognizes Mouse FGF-9. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Fibroblast growth factor 9 (glia-activating factor), also known as FGF9 is a glycosylated neurotrophic polypeptide highly expressed in brain.1 It is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. FGF-9 has sequence similarity of approximately 30% to other members of the family of fibroblast growth factors.2 It is highly related to FGF-16.
FGF-9 has been shown to mediate its effects by binding to FGF receptors. It efficiently activates the FGFR2c splice form of FGFR2 and the FGFR3b and FGFR3c splice isoforms of FGFR3.3 FGF-9 is a high affinity, heparin dependent ligand for FGFR3 and FGFR2 but not for FGFR1 and FGFR4.4
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Mouse FGF-9 (>98%)
Formulation
This antigen affinity purified polyclonal antibody is aseptically filtered and lyophilized from PBS with no carrier proteins or preservatives added.
Reconstitution
Aseptically reconstitute this antigen affinity purified polyclonal antibody using 100 µl of sterile deionized water.
Storage and Stability
The lyophilized antibody should be stored desiccated at -20°C. The reconstituted antibody can be stored for at least six weeks at 4°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 µg/ml with a suitable second step reagent such as Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115). The detection sensitivity of this indirect ELISA for Mouse FGF-9 is approximately 0.4 ng/well.
Neutralization: Antibody concentration of 0.06 ug/mL was used to yield one-half maximal inhibition (ED50) of the biologic activity of 1.5 ng/mL mouse FGF-9.
Western Blotting: To detect Mouse FGF-9 this polyclonal antibody can be used at a concentration of 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse FGF-9 is 1.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Imamura T. et al. (1999)J Biol Chem 274: 29352
2. Miyamoto M et al. (1993)Molecular Cellular Biology 13: 4251
3. Santos-Ocampo S et al. (1996)Journal of Biological Chemistry 271: 1726
4. Hecht D et al. (1995)Growth Factors 12: 223
Related Products
Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse FGF-9-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: F132)
Anti-Human FGF-9 (Leinco Prod. No.: F1070)
Anti-Human FGF-9-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: F1093)
Recombinant Human FGF-9 (Leinco Prod. No.: F123)
Recombinant Mouse FGF-9 (Leinco Prod. No.: F111)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
