产品编号:R139
产品描述:Anti-Human sRANKL
反应种属:human
实验方法:ELISA, ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Rabbit Anti-Human Soluble Receptor Activator of NF-KappaB Ligand (sRANKL)
Antigen Affinity Purified
(CD254, TRANCE, hRANKL2, sOdf, TNF-Related Activation-Induced Cytokine [TRANCE], Osteoprotegrin Ligand [OPGL], Osteoclast Differentiation Factor [ODF], TNFSF11)
Polyclonal Antibody
Prod. No.: R139
Host: Rabbit
Pkg. Size: 1 mg, 100 µg
Storage: -20°C
Description
Specificity:
Rabbit Anti-Human Soluble Receptor Activator of NF-KappaB Ligand (sRANKL) recognizes Human sRANKL. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
RANKL (Receptor Activator for Nuclear Factor κ B Ligand), also known as TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and ODF (osteoclast differentiation factor), is a member of the tumor necrosis factor family cytokine.1 RANKL is essential for osteoclast formation, activation, and survival.2 RANKL through interaction with its receptor RANK can modulate matrix degradation and inflammation.3 RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy. Consequently, novel drugs specifically targeting RANK, RANKL, and their signaling pathways in osteoclasts are expected to revolutionize the treatment of various ailments associated with bone loss, such as arthritis, periodontal disease, cancer metastases, and osteoporosis.4
Immunogen
Purified Recombinant sRANKL (>98%)
Formulation
This antigen affinity purified polyclonal antibody is aseptically filtered and lyophilized from PBS with no carrier proteins or preservatives added.
Reconstitution
Aseptically reconstitute this antigen affinity purified polyclonal antibody using sterile deionized water to a concentration >0.2 mg/ml.
Storage and Stability
The lyophilized antibody should be stored desiccated at -20°C. The reconstituted antibody can be stored for at least six weeks at 4°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
ELISA Cap, Indirect ELISA, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5 µg/ml. A suitable detection antibody is Anti-Human sRANKL-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: R149) at a concentration of approximately 0.2 µg/ml. When used in conjunction with an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" (Leinco Prod. No.: T118), the sensitivity of this sandwich ELISA is 0.4 ng/well.
Western Blotting: To detect Human sRANKL this polyclonal antibody can be used at a concentration of 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human sRANKL is 1.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. H Takayanagi et al. (2003) Arthritis Res Ther. 5: 20
2. Kostenuik PJ et al. (2005) Endocrinology 146: 3235
3. Holven KB et al. (2009) Stroke 40: 241
4. Penninger JM et al. (2008) Ann N Y Acad Sci. 1143: 123
Related Products
Anti-Human sRANKL-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: R149)
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" (Leinco Prod. No.: T118)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Human sRANKL ELISA Development Kit (Leinco Prod. No.: R144)
Anti-Human sRANKL (Monoclonal Antibody) (Leinco Prod. No.: R1138)
Anti-Mouse sRANKL-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: R1145)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
