产品编号:S115
产品描述:Anti-Human SDF-1β
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5595.00
订购:
说明书:
Goat Anti-Human Stromal Cell-Derived Factor-1 Beta (SDF-1 β)
Antigen Affinity Purified
(Thymic Lymphoma Cell Stimulating Factor-Beta (TLSF-b), CXCL12b, Pre-B Cell Growth-Stimulating Factor (PBSF))
Polyclonal Antibody
Prod. No.: S115
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Stromal Cell-Derived Factor-1 Beta (SDF-1 β) recognizes Human SDF-1 β. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
SDF-1 beta (stromal cell-derived factor-1 beta) is a non-glycosylated Polypeptide that is produced in E-Coli, belonging to the alpha chemokine (C-X-C) family of cytokines.1 SDF-1beta is a highly efficacious chemoattractant for resting lymphocytes and CD34(+) progenitor cells, and it efficiently blocks the CXCR-4-mediated entry into cells of T cell line tropic strains of HIV type 1 (HIV-1). SDF-1beta promotes HIV-1 replication and disease progression.2
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human SDF-1 β (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
References
1. Muller HW et al. (2000) Eur J Neurosci. 12: 1857
2. Nagai Y et al. (1998) Proceedings of National Acad Sci USA 95: 6331
Related Products
Recombinant Mouse SDF-1β (Leinco Prod. No.: S502)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
