产品编号:C220
产品描述:Anti-Human sCD22
反应种属:human
实验方法:ELISA, ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5865.00
订购:
说明书:
Rabbit Anti-Human Soluble Cluster of Differentiation 22 (sCD22)
Antigen Affinity Purified
(BL-CAM, MGC130020, SIGLEC2)
Polyclonal Antibody
Prod. No.: C220
Host: Rabbit
Pkg. Size: 1 mg, 100 µg
Storage: -20°C
Description
Specificity:
Rabbit Anti-Human Soluble CD22 (sCD22) recognizes Human sCD22. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Antigen Distribution:
Human CD22 is present in the cytoplasm of nearly all B-lineage cells and is also expressed on the surface of B-cells during advance stages of differentiation.
Background:
Cluster of differentiation 22 also known as CD22 is a B-cell-restricted transmembrane protein, which acts as a negative regulator of B-cell signalling. CD22 also has lectin-like adhesive properties.1 CD22 is a good target to shorten the time required for Ab production, thereby augmenting host defense against acute infectious diseases as "universal vaccination."2
Immunogen
Purified Recombinant Human sCD22 (>98%)
Formulation
This antigen affinity purified polyclonal antibody is aseptically filtered and lyophilized from PBS with no carrier proteins or preservatives added.
Reconstitution
Aseptically reconstitute this antigen affinity purified polyclonal antibody using sterile deionized water to a concentration >0.2 mg/ml.
Storage and Stability
The lyophilized antibody should be stored desiccated at -20°C. The reconstituted antibody can be stored for at least six weeks at 4°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
ELISA Cap, Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115). The detection sensitivity of this indirect ELISA for Human sCD22 is approximately 0.4 ng/well.
Western Blotting: To detect Human sCD22 this polyclonal antibody can be used at a concentration of 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human sCD22 is 1.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Crocker PR et al. (2000) Immunol. 101: 342
2. Tsubata T et al. (2008) J Immunol. 180: 907
Related Products
Goat Anti-Rabbit IgG (H&L) Horseradish Peroxidase (Leinco Prod. No.: R115)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human sCD22-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C221)
Recombinant Human CD22 (Extracellular Domain) (Leinco Prod. No.: C177)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
