产品编号:R1142
产品描述:Anti-Human Resistin
反应种属:human
实验方法:ELISA, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Resistin (RETN)
Antigen Affinity Purified
(Adipocyte-Specific Secretory Factor (ADSF), Inflammatory Zone 3 (FIZZ3), MGC126603, MGC126609, RETN1, RSTN, CEBPE Regulated Myeloid-Specific Secreted Cysteine-Rich Protein Precursor 1 (XCP1))
Polyclonal Antibody
Prod. No.: R1142
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Resistin recognizes Human Resistin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Resistin (RETN) is a peptide hormone secreted by adipocytes 1 with potential roles in insulin resistance and adipocyte differentiation.2 Resistin functions as a regulator of glucose homeostasis and a physiologic antagonist to hepatic insulin action.3 Resistin may be an important candidate mediator of obesity-induced insulin resistance.4 It may also represent a novel link between metabolic signals, inflammation, and atherosclerosis.5
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human Resistin (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 0.5 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.2 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
References
1. Lazar MA et al. (2001) J Biol Chem. 276: 25970
2. Lazar MA et al. (2002) J Biol Chem. 277: 19754
3. Wolf G et al. (2004) Nutr Rev. 62: 389
4. Chen R et al. (2004) Eur J Endocrinol. 150: 885
5. Rader DJ et al. (2005) Circulation 111: 9
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
