产品编号:P121
产品描述:Anti-Human PDGF-AA
反应种属:human
实验方法:ELISA, IHC, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5595.00
订购:
说明书:
Goat Anti-Human Platelet Derived Growth Factor-AA (PDGF-AA )
Antigen Affinity Purified
(Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF))
Polyclonal Antibody
Prod. No.: P121
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Platelet Derived Growth Factor-AA (PDGF-AA ) recognizes Human PDGF-AA . This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Platelet-derived growth factor (PDGF), an agent with important mitogenic effects for bone cells, exists in three isoforms, PDGF-AA, - BB, and –AB.1 The A-chain homodimers of the platelet-derived growth factor (PDGF AA) are widely expressed in normal and transformed cells.2 PDGF AA is a potent mitogen for a wide range of cell types including fibroblast, smooth muscle, and connective tissue. PDGF-AA and its receptor represent an important epithelial-mesenchymal interaction which plays a critical role in early lung branching morphogenesis.3 PDGF AA is also an essential autocrine regulator for vascular endothelial growth factor (VEGF) expression. It regulates the frequency and level of VEGF expression during the transition from a precancerous lesion to advanced cancer.4
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human PDGF-AA (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
References
1. E Canalis et al. (1994) Endocrin. 134: 2541
2. Deuel TF et al. (1992) Biochem Biophys Res Commun. 186: 1510
3. M Post et al. (1995) Endocrin. 121: 25529
4. Katsuo Sueishi et al. (2005) Cancer Research 65: 7241
Related Products
Anti-Human PDGF-AA (Leinco Prod. No.: P274)
Recombinant Mouse PDGF-AA (Leinco Prod. No.: P297)
Anti-Human PDGF-AA-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: P124)
Recombinant Human PDGF-AA (Leinco Prod. No.: P125)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
