产品编号:N113
产品描述:Anti-Human NT-3
反应种属:human
实验方法:ELISA, IHC-F, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5595.00
订购:
说明书:
Goat Anti-Human Neurotrophin-3 (NT-3)
Antigen Affinity Purified
(Nerve Growth Factor-2 (NGF-2), HGN NTF3, HDNF, MGC129711)
Polyclonal Antibody
Prod. No.: N113
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Neurotrophin-3 (NT-3) recognizes Human NT-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Neurotrophin 3 (NT-3), also known as NTF3 is a basic protein found in neurons of the central nervous system. NT-3 selectively supports the survival of neuronal cell populations. It rapidly potentiate the spontaneous and impulse-evoked synaptic activity of developing neuromuscular synapses in culture and thus appears to be involved in the regulation of functions of developing synapses.1,2,3 NT-3 negatively modulates nerve growth factor (NGF) receptor expression and associated nociceptive phenotype in intact neurons, suggesting a beneficial role in treating aspects of neuropathic pain mediated by NGF. NT-3 is effective at suppressing thermal hyperalgesia associated with chronic constriction injury (CCI).4
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human NT-3 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<10 ng/1 mg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (Frozen), N, WB
References
1. Barde YA. et al. (1990) Progress in Growth Factor Res. 2: 237
2. Thoenen H. et al. (1991) Trends in Neurological Scienc. 14: 165
3. Vantini G. et al. (1992) Psychoneuroendocrinology 17: 401
4. Valerie M. K. Verge et al. (2005) J of Neuroscie. 25: 758
Related Products
Recombinant Human NT-3 (Leinco Prod. No.: N117)
Anti-Human NT-3 (Leinco Prod. No.: N200)
Anti-Human NT-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: N128)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
